PSII-5 Endotoxin-Induced Inflammation on Immune Markers and Steroidogenesis in Granulosa Cells

Abstract Reproductive performance is central to profitability in beef cattle operations. Pathogenic stress can result from gram-negative bacteria release of the lipopolysaccharide (LPS) endotoxin which has potential accumulate follicular fluid and impair folliculogenesis steroidogenesis, leading reduced fertility. Elevated LPS concentrations observed acute clinical bacterial infections are recognized have detrimental effects on female fertility, however, definitive low present subclinical or undetectable infection remains be fully elucidated. This study investigated effect varying mechanisms regulating ovarian function subsequent steroidogenesis. A granulosa cell line (KGN) was cultured with without increasing doses (0.0001 10 µg/mL) for 30 min 48 h. Cells collected after treatment were analyzed abundance phosphorylated proteins cells h total protein gene expression. Media by radioimmunoassay steroid hormone concentrations. Quantitative semi-quantitative PCR used evaluate aromatase, beta-catenin, cytokines IL-6 IL-8, respectively. No differences detected among groups compared vehicle-treated controls beta-catenin (P = 0.99) active lacking phosphorylation at serine 33/37 0.95) evaluated Similar results forms 552 0.98) 675 0.85) min. Additionally, estradiol not different 0.0001 1.0 µg/mL LPS; significantly decreased control 0.03). Progesterone also similar within a tendency decrease 0.08) control. Varying did change mRNA expression 0.28), aromatase 0.32), 0.35). However, significant increase cytokine IL-8 0.04). These findings suggest that inflammatory response against higher may influence distinct Conversely, basal key steroidogenic enzymes modulators less affected LPS. Therefore, immune dependent concentration exposure ability impact fertility modulating hormonal environment.